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Biblioteca (s) : |
INIA Treinta y Tres. |
Fecha : |
05/04/2022 |
Actualizado : |
01/09/2022 |
Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
Autor : |
SCHVARTZMAN, C.; FRESIA, P.; MURCHIO, S.; MUJICA, V.; DALLA RIZZA, M. |
Afiliación : |
CLAUDIA SCHVARTZMAN DISEGNI, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; PABLO FRESIA, Unidad Mixta UMPI, Institut Pasteur Montevideo + INIA, Montevideo, Uruguay; MARIA SARA MURCHIO VIGNOLO, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; MARIA VALENTINA MUJICA TELIZ, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; MARCO DALLA RIZZA VILARO, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay. |
Título : |
RNAi in Piezodorus guildinii (Hemiptera: Pentatomidae): transcriptome assembly for the development of pest control strategies. |
Fecha de publicación : |
2022 |
Fuente / Imprenta : |
Frontiers in Plant Science, 01 April 2022, volume13, article 804839. OPEN ACCESS. Doi: https://doi.org/10.3389/fpls.2022.804839 |
Páginas : |
13 p. |
DOI : |
10.3389/fpls.2022.804839 |
Idioma : |
Inglés |
Notas : |
Article history: Received: 29 October 2021; Accepted: 28 February 2022; Published: 01 April 2022.
Correspondence: Marco Dalla-Rizza, mdallarizza@inia.org.uy
Al abrir el registro se visualiza el enlace al material suplementario. |
Contenido : |
Red-banded stink bug Piezodorus guildinii (P. guildinii) has been described as the most damaging stink bug regarding soybean crops, leading to seed injury, low germination percentages, and foliar retention, at low population densities. In recent years, RNA interference (RNAi), a conserved eukaryote silencing mechanism has been explored to develop species-selective pesticides. In this work, we evaluated RNAi in P. guildinii to develop new pest-control strategies. For this, we assembled and annotated a P. guildinii transcriptome from a pool of all developmental stages. Analysis of this transcriptome led to the identification of 56 genes related to the silencing process encompassing siRNA, miRNA, and piRNA pathways. To evaluate the functionality of RNAi machinery, P. guildinii adults were injected with 28 ng/mg of body weight of double stranded RNA (dsRNA) targeting vATPase A. A mortality of 35 and 51.6% was observed after 7 and 14 days, respectively, and a downregulation of vATPase A gene of 84% 72 h post-injection. In addition, Dicer-2 and Argonaute-2 genes, core RNAi proteins, were upregulated 1.8-fold 48 h after injection. These findings showed for the first time that RNAi is functional in P. guildinii and the silencing of essential genes has a significant effect in adult viability. Taken together, the work reported here shows that RNAi could be an interesting approach for the development of red-banded stink bug control strategies. |
Palabras claves : |
DSRNA,; PEST CONTROL; RNA-SEQ; RNAI; STINK BUG; VATPASE A,. |
Asunto categoría : |
H10 Plagas de las plantas |
URL : |
http://www.ainfo.inia.uy/digital/bitstream/item/16649/1/Frontiers-Plant-Science-2022-Schvartzman.pdf
http://www.ainfo.inia.uy/digital/bitstream/item/16650/1/Frontiers-Plant-Science-2022-Schvartzman-1.PDF
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Marc : |
LEADER 02536naa a2200277 a 4500 001 1062960 005 2022-09-01 008 2022 bl uuuu u00u1 u #d 024 7 $a10.3389/fpls.2022.804839$2DOI 100 1 $aSCHVARTZMAN, C. 245 $aRNAi in Piezodorus guildinii (Hemiptera$bPentatomidae): transcriptome assembly for the development of pest control strategies.$h[electronic resource] 260 $c2022 300 $a13 p. 500 $aArticle history: Received: 29 October 2021; Accepted: 28 February 2022; Published: 01 April 2022. Correspondence: Marco Dalla-Rizza, mdallarizza@inia.org.uy Al abrir el registro se visualiza el enlace al material suplementario. 520 $aRed-banded stink bug Piezodorus guildinii (P. guildinii) has been described as the most damaging stink bug regarding soybean crops, leading to seed injury, low germination percentages, and foliar retention, at low population densities. In recent years, RNA interference (RNAi), a conserved eukaryote silencing mechanism has been explored to develop species-selective pesticides. In this work, we evaluated RNAi in P. guildinii to develop new pest-control strategies. For this, we assembled and annotated a P. guildinii transcriptome from a pool of all developmental stages. Analysis of this transcriptome led to the identification of 56 genes related to the silencing process encompassing siRNA, miRNA, and piRNA pathways. To evaluate the functionality of RNAi machinery, P. guildinii adults were injected with 28 ng/mg of body weight of double stranded RNA (dsRNA) targeting vATPase A. A mortality of 35 and 51.6% was observed after 7 and 14 days, respectively, and a downregulation of vATPase A gene of 84% 72 h post-injection. In addition, Dicer-2 and Argonaute-2 genes, core RNAi proteins, were upregulated 1.8-fold 48 h after injection. These findings showed for the first time that RNAi is functional in P. guildinii and the silencing of essential genes has a significant effect in adult viability. Taken together, the work reported here shows that RNAi could be an interesting approach for the development of red-banded stink bug control strategies. 653 $aDSRNA, 653 $aPEST CONTROL 653 $aRNA-SEQ 653 $aRNAI 653 $aSTINK BUG 653 $aVATPASE A, 700 1 $aFRESIA, P. 700 1 $aMURCHIO, S. 700 1 $aMUJICA, V. 700 1 $aDALLA RIZZA, M. 773 $tFrontiers in Plant Science, 01 April 2022, volume13, article 804839. OPEN ACCESS. Doi: https://doi.org/10.3389/fpls.2022.804839
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Registro original : |
INIA Treinta y Tres (TT) |
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Biblioteca (s) : |
INIA Las Brujas. |
Fecha actual : |
20/01/2022 |
Actualizado : |
20/01/2022 |
Tipo de producción científica : |
Trabajos en Congresos/Conferencias |
Autor : |
CAPELESSO, A.; MENDOZA, A.; KOZLOSKI, G.; CAJARVILLE, C.; DAYUTO, J.; REPETTO, J. |
Afiliación : |
A. CAPELESSO, Universidad de la República, Facultad de Veterinaria, Uruguay; Universidade Federal de Santa Maria, Brasil.; ALEJANDRO FRANCISCO MENDOZA AGUIAR, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; G. KOZLOSKI, Universidade Federal de Santa Maria, Brasil.; CECILIA CAJARVILLE, Universidad de la República, Facultad de Veterinaria, Uruguay.; J. DAYUTO, Universidad de la República, Facultad de Veterinaria, Uruguay.; JOSÉ REPETTO, Universidad de la República, Facultad de Veterinaria, Uruguay. |
Título : |
Extra walking of dairy cows to access the pasture in a grazing system: Effect on feeding behaviour. |
Fecha de publicación : |
2021 |
Fuente / Imprenta : |
In: AUPA, Proceedings del VII Congreso Uruguayo de Producción Animal. Sección Cambio Climático y Producción Sostenible (Climate Change Section), 14 y 15 diciembre 2021. Archivos Latinoamericanos de Producción Animal, 29(Supl.1), p.21-23. |
Serie : |
(Archivos Latinoamericanos de Producción Animal, Vol.29, Supl.1) |
ISSN : |
1022-1301; online 2075-8359 |
Idioma : |
Inglés |
Contenido : |
The objective of this study was to investigate the feeding behaviour impact due to walking in dairy cowswalking 5 km between the milking parlour and the pasture plot in a grazing system, separating the effect of walking itself from the fasting period associated to walking. |
Palabras claves : |
Milk yield; Pasture dairy system; Short fasting. |
Asunto categoría : |
F01 Cultivo |
URL : |
http://www.ainfo.inia.uy/digital/bitstream/item/16199/1/2950-Article-Text-10068-5-10-20211213-06.pdf
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Marc : |
LEADER 01209nam a2200229 a 4500 001 1062656 005 2022-01-20 008 2021 bl uuuu u01u1 u #d 022 $a1022-1301; online 2075-8359 100 1 $aCAPELESSO, A. 245 $aExtra walking of dairy cows to access the pasture in a grazing system$bEffect on feeding behaviour.$h[electronic resource] 260 $aIn: AUPA, Proceedings del VII Congreso Uruguayo de Producción Animal. Sección Cambio Climático y Producción Sostenible (Climate Change Section), 14 y 15 diciembre 2021. Archivos Latinoamericanos de Producción Animal, 29(Supl.1), p.21-23.$c2021 490 $a(Archivos Latinoamericanos de Producción Animal, Vol.29, Supl.1) 520 $aThe objective of this study was to investigate the feeding behaviour impact due to walking in dairy cowswalking 5 km between the milking parlour and the pasture plot in a grazing system, separating the effect of walking itself from the fasting period associated to walking. 653 $aMilk yield 653 $aPasture dairy system 653 $aShort fasting 700 1 $aMENDOZA, A. 700 1 $aKOZLOSKI, G. 700 1 $aCAJARVILLE, C. 700 1 $aDAYUTO, J. 700 1 $aREPETTO, J.
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